Production of Erythropoietin-Specifi c Polyclonal Antibodies
نویسندگان
چکیده
منابع مشابه
Production of Erythropoietin-specific polyclonal Antibodies
Background: Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantification of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require sp...
متن کاملProduction of Erythropoietin-Specific Polyclonal Antibodies
BACKGROUND Erythropoietin, as a principal hormone promotes red blood cell production in bone marrow. Varieties of erythropoietin biosimilar are being produced by recombinant DNA technology in cell cultures. The detection or quantifi cation of these molecules are being performed by diff erent methods which some of theme such as Western blot and enzymelinked immunosorbent assay (ELISA) require sp...
متن کاملProduction of polyclonal antibodies in rabbits.
The generation of polyclonal antibodies to an antigen of interest is an important technique applicable to many areas of biological research. In this chapter, we describe a basic immunization procedure designed to generate polyclonal antisera in rabbits and two methods that are commonly employed in the subsequent preliminary characterization of antipeptide antibodies raised in this way.
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diphtheria is a fatal disease caused by exotoxin of corynebacterium diphtheria . this toxin consists of two chains, catalytic chain (a) and binding (b) chain. by binding chain (b), the toxin binds to its receptor on numerous body cells such as myocardial, kidney and peripheral nerve cells. after entering, catalytic chain (a) inhibits protein synthesis and finally can cause cell dea...
متن کاملSingle-Batch Production of Recombinant Human Polyclonal Antibodies
We have previously described the development and implementation of a strategy for production of recombinant polyclonal antibodies (rpAb) in single batches employing CHO cells generated by site-specific integration, the Sympress I technology. The Sympress I technology is implemented at industrial scale, supporting a phase II clinical development program. Production of recombinant proteins by sit...
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ژورنال
عنوان ژورنال: Iranian Journal of Biotechnology
سال: 2017
ISSN: 1728-3043,2322-2921
DOI: 10.15171/ijb.1413